Regulatory
SV40(en)

Part:BBa_K1722006:Design

Designed by: Zeyu Miao   Group: iGEM15_SZU_China   (2015-09-01)


SV40（with Enhancer） is a widely used strong promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed the following primers and amplified SV40 promoter from the vector psi-Check2:

By incorporating these primers into hUPII promoter, the promoter is flanked by the iGEM prefix and suffix after amplification.


Source

SV40 promoter with enhancer was achieved from Shenzhen Second People's Hospital.

References

[1] Ouy C, Gardnert A, Kao C, et al. A potential of tissue restrictive gene therapy in renal cell carcinoma using MN/CA IX promoter[J]. Anticancer Res, 2005, 25(2A): 881-886

[2] Pu J, Yang Q, Guo D, et al. Effects of nuclear factor of activated T cells on the promoter activity of the constitutively activated SV40[J]. Chinese Journal of Cellular and Molecular Immunology, 2012,28(5): 452-457

[3] Liu QJ, Yin YJ, Wang B, et al. The Activity of SV40 promoter can be inhibited by overexpression of Hemeoxygenase-1 in tumor cells[J]. Cell Biochem Biophys, 2013, 65(3): 287-295

[4] Mcelroy D, Brettell R. Foreign gene expression in transgenic cereals[J]. Trends, Biotechnol, 1994,12(2): 62-68